PhD Studentship: Molecular mechanisms of membrane proteins

Design and Characterization of Bio-inspired Nanosensors Engineered by Assembly of

Receptors and Ion Channels

The subject. We have devised a novel type of bio-inspired sensors, Ion-Channel Coupled Receptors

(ICCR), based on the fusion of receptors with an ion channel serving as an electrical probe. Binding of

a ligand to the receptor triggers opening of the associated ion channel and an increased ion flux that is

easily detectable using existing techniques. This concept was validated by the creation of ICCRs

between a potassium channel and several G-protein coupled receptors (GPCR) [cf. Moreau et al, 2008,

Nature Nanotech 3:620-5].

The thesis shall concern the structure-function study of receptor-channel interactions in existing ICCRs

and on the design and construction of new ICCRs. Work will involve molecular engineering, biophysics,

and bioinformatics. It should establish the foundation of high-performance bioelectrical sensors while

providing a better understanding of the molecular mechanisms of GPCR activation and ion channel

gating.

Although this project has a strong biotechnological flavor, the approach shall remain very fundamental

with a strong focus on the structure and function of membrane proteins and on their interactions.

The place. The thesis will take place in the Channels lab within the Membrane Proteins Dept of the

Institute for Structural Biology (www.ibs.fr/laboratories/membrane-protein-lab-lpm/transportersreceptors-

and-ion). The Channels lab focuses on ion channels and membrane protein engineering and

is recognized for its work on K-ATP channel and proteic biosensors.

IBS is a renowned institute in the field of structural and molecular biology. It is part of the Grenoble

scientific polygon with numerous research intitutes and colleges as well as several large instruments

such as the European Synchrotron. The site offers a stimulating scientific environment in the natural

surroundings of the French Alps.

The candidate. Strong academic background. Experience or interest in molecular biology,

electrophysiology, or bioinformatics.

The granting agency (CEA) will only consider candidates with top-tier academic records. There are no

other restrictive conditions and foreigners can, and should, apply.

The position. The position is for three years starting in October 2010. The salary is very competitive

(~2000 €/month).

Send a one-page CV to Michel Vivaudou (vivaudou@cea.fr). A full-fledged application will be

requested subsequently.